Fig. 5. Trichome differentiation in CAF-1 mutants. (A,B) Trichomes from leaves of wild-type (Col) plants usually have three branches (A), whereas trichomes on fas1-4 leaves develop up to six branches (B). (C-F) Trichomes of wild-type (Col) and fas1-4 plants are single cells with one nucleus. (C,D) DAPI-stained trichomes of wild type (Col) and fas1-4, respectively. (E,F) Close-up view of nuclei in DAPI-stained trichomes of wild type (Col) and fas1-4, respectively. (G) Trichome branching for the first two rosette leaves of at least six plants per experiment. Shown are the average values of several independent experiments. Segments represent fractions of trichomes with 1 (white hatched), 2 (white), 3 (light gray), 4 (dark gray), 5 (black) and 6 (dark gray, hatched) branches. (H) Ploidy of trichome nuclei from the first two rosette leaves. DNA in trichome nuclei was stained with DAPI and the fluorescence intensity values were normalized to the fluorescence of guard cell nuclei from the same specimen; these are displayed relative to wild-type intensities. Shown are the average values of several independent experiments (means±s.e.m.). Double asterisks indicate P-values (t-test) <0.005.