Fig. 8. EGL-38 and PAX-2 directly regulate ced-9 transcription in vivo. (A) Diagram of the mev-1 ced-9 genomic region, showing the position of primers used for chromatin immunoprecipitation. Black arrowheads correspond to primers for the upstream region (UP); white arrowheads correspond to primers for the coding region (CR). (B) PCR of DNA recovered from chromatin immunoprecipitation. Input lane corresponds to DNA recovered without immunoprecipitation. Two representative experiments are shown, one each for a strain expressing EGL-38 (hsp::egl-38::FLAG) and PAX-2 (hsp::pax-2::FLAG). The experimental proteins (EGL-38 and PAX-2) are tagged at the C-terminus with a FLAG peptide (Fig. 2B), and can be immunoprecipitated with an -FLAG antibody. PCR of DNA recovered from immunoprecipitation using -FLAG antibody, protein A/G beads (negative control) or -Histone (positive control) are shown for representative experiments using EGL-38 (left) and PAX-2 (right). In both cases, the Pax2/5/8 protein preferentially immunoprecipitates DNA in the upstream region.