Fig. 4. ARF6 expression, and flower and ovule phenotypes of
mARF6 plants. (A) Northern blot analysis of ARF6
transcript levels in wild-type, arf6-2, P35S::ARF6, and
individual mARF6 and gARF6 transgenic plant flowers. The
transcript of P35S::ARF6 is shorter because it lacks the
5' and 3' UTRs. Arrow indicates the ARF6 transcript.
Numbers beneath lanes indicate relative ARF6 transcript levels
normalized to the ß-tubulin loading control. (B) Wild-type,
gARF6, mARF6-I and mARF6-II flowers. Arrows indicate
indehiscent anthers. (C-E,G-I) Wild-type (C-F) and
mARF6-II (G-J) stage 2-IV (C,G) and stage 4-I (D,E,H,I) ovules.
Arrows in C,D,G and H indicate outer integuments. (K) Stage 4-I
mARF6-I ovule. (L) Stage 4-I mARF6-III ovule;
asterisk indicates exposed embryo sac. (F,J,M) Wild-type
(F,M left), arf6 arf8 (M middle) and mARF6-II (J,M right)
gynoecia (M) and ovules (F,J) after pollination with the pollen-specific
reporter LAT52:GUS pollen
(Johnson et al., 2004).
(N,O) Embryos of gARF6 (N) and mARF6-II (O)
plants 7 days after pollination with wild-type pollen. Arrowhead in O
indicates arrested embryo. fu, funiculus; ii, inner integument; oi, outer
integument; nu, nucellus. Scale bars: 0.3 mm in B,M; 12 µm in
C,D,F,G,H,J-L,N,O; 20 µm in E,I.