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Figure 2


Fig. 2. Subcellular localization of Profilin1 and Daam1 in response to Wnt stimulation. (A) NIH3T3 cells respond to Wnt3a and Wnt5a but not control conditioned media (CM). After 3 hours of exposure of cells to Wnt3a CM or Wnt3a protein, endogenous ß-catenin (red) is localized in the nucleus and stress fiber induction is indicated by Phalloidin staining (green). After 3 hours of exposure to Wnt5a CM, no nuclear localization of ß-catenin is seen but stress fiber induction is observed. Position of the nucleus is indicated by arrow. (B) Expression of Flag-{Delta}DIX-Dishevelled (red) induces stress fiber formation (green) in NIH3T3 cells. (C) Subcellular localization of GFP-Profilin1 in NIH3T3 cells. GFP-Profilin1 is localized diffusely in the cytoplasm of NIH3T3 cells in the presence of control CM but associates with actin stress fibers (arrow) (red) after 3 hours Wnt-3a CM stimulation. (D) Endogenous Daam1 (red) is localized diffusely in the cytoplasm of NIH3T3 cells but is localized to actin stress fibers (arrow) (green) after 3 hours Wnt3a CM stimulation. (E) Quantification of the studies of C and D. Number of cells evaluated is shown at the top of each bar. Note in B,C,D magnifications of boxed areas (white) are shown below each respective panel.