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Figure 4


Fig. 4. Profilin1 is required for Wnt3a and Dishevelled-mediated stress fiber induction. (A) Transfection of Profilin1 siRNA but not GFP siRNA into NIH3T3 cells inhibits stress fiber induction (green) by 3 hours Wnt3a CM or Wnt3a protein stimulation. Note the Profilin1 siRNA does not inhibit stress fiber induction induced by serum stimulation. Nuclear translocation of endogenous ß-catenin (red) induced by Wnt3a CM or purified Wnt3a protein stimulation is not affected by Profilin1 siRNA. (B) Transfection of Profilin1 siRNA but not GFP siRNA into NIH3T3 cells inhibits stress fiber induction (green) induced by {Delta}DIX-Dishevelled expression (red), magnifications of the boxed areas (white) are shown at the side of each respective panel. (C) Quantification of the results of A and B. Number of cells evaluated is shown at the top of each bar.