Fig. 5. Profilin1 is required for Daam1-mediated stress fiber induction. (A) Expression of Myc-tagged C-Daam1 (red) in NIH3T3 cells induces stress fibers (green), and this effect is abrogated by coexpression of Profilin1 siRNA but not GFP siRNA, magnifications of the boxed areas (white) are shown at the side of each respective panel. (B) Quantification of the effects on Profilin1 and GFP siRNA on stress fiber formation induced by C-Daam1 expression in NIH3T3 cells. Number of cells evaluated is shown at the top of each bar.