Fig. 2. Pseudoglandular development in Cebpa knockout mouse livers. Hematoxylin and Eosin staining indicates that periportal hepatoblasts form pearl-like structures in 14.5-day and 17.5-day wild-type livers (arrowheads) (A-C), while pseudoglandular or pearl-like structures are abundant in the whole liver parenchyma of knockout livers (small arrows) (E-G). Biliary epithelial cells differentiate only around the portal vein and a typical intrahepatic bile duct is formed in a neonatal wild-type liver (D). In a neonatal knockout liver, pseudoglandular structures with large lumina still abundantly develop and no normal bile duct is seen even around the portal vein (H). HV, hepatic vein; PV, portal vein. Scale bars: 50 µm.