Fig. 6. Higher proliferation of pseudoglandular cells in the knockout mouse liver. (A-D) PCNA immunostaining shows an increase of proliferation in pseudoglandular cells in 17.5-day (C) and neonatal (D) knockout mouse livers (arrowheads), compared with wild-type mice (arrows, A,B). PV, portal vein. Scale bars: 50 µm. Proliferation indexes of hepatic cells, given as number of PCNA-positive cells per portal biliary epithelial cell (light brown) or nonperiportal hepatocyte/pseudoglandular cell (purple), show a decrease and increase of proliferation in periportal biliary epithelial cells and nonperiportal pseudoglandular cells of the knockout mouse liver, respectively, compared with wild-type mice (E; wild type, n=3; knockout, n=3). Bars indicate the standard deviations of the means.