Fig. 3. DPPIV+ hepatocyte colonies in livers of DPPIV-host rats transplanted with CMP-FLEC. Acetone fixed, frozen sections from livers of DPPIV-, retrorsine/PH treated host rats at 3 months after transplantation of CMP-FLEC_II-IV were stained with Hematoxylin and Eosin (A-C) or histochemically for DPPIV (D-J). Sections in A-C were prepared from liver tissue harvested from the same animal used in D. Long and short arrows in A indicate, respectively, an inflammatory focus and two megalocytes. (B) Arrows delineate megalocytes and the line traces the margin of a small hepatocyte colony. (C) Arrows identify a typical portal area with minimal oval cell proliferation. (D-I) Hepatocyte colonies with canalicular DPPIV activity in host rat livers transplanted with (D) CMP-FLEC_II,III, isolate R; (E) CMP-FLEC_II, isolate D; (F) CMP-FLEC_IV, isolate P; (G) total ED16 fetal liver, isolates A and F; (H) CMP-FLEC_III, isolate B; (I) CMP-FLEC_I isolate U. (J) Canalicular pattern of DPPIV activity in histochemically stained, acetone-fixed frozen section from an adult DPPIV+ rat liver. Scale bars: 200 µm.