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Figure 8


Fig. 8. Expression of hepatocyte associated proteins by DPPIV+ colonies derived from CMP-FLEC. Acetone-fixed frozen sections were stained by double label IIF. (A,B) DPPIV+ (green canalicular fluorescence) donor-derived hepatocyte colonies present 9 months after transplantation of CMP-FLECIV (Table 1, isolate P) were strongly positive for hepatocyte markers H.4 (A, red) and H.1 (B, red). (C) 3 month DPPIV+ colony (green) derived from CMP-FLECII (isolate D) showing positive reactivity with a MAb specific for CYP2E1 (red). Scale bars: 100 µm.