Fig. 5. Polarity defects in pam-1 mutants occur in the absence of meiotic exit defects. (A-D) DIC images of cyb-3(RNAi) (A,C) and pam-1; cyb-3(RNAi) (B,D) embryos. cyb-3(RNAi) embryos show normal pseudocleavage (A) and a posteriorly displaced spindle (C), while many pam-1; cyb-3(RNAi) embryos lack pseudocleavage (B) and have a centrally positioned spindle (D). (E-I) Triple-stained confocal images with PGL-1 staining of P granules in red, tubulin staining in green and DAPI DNA staining in blue. While P granules were not localized in meiotic embryos of cyb-3(RNAi) and pam-1; cyb-3(RNAi) (E-F), they were localized to the posterior in all cyb-3(RNAi) embryos during the first mitosis (G). P granules failed to localize posteriorly in most pam-1; cyb-3(RNAi) embryos, being distributed either throughout the cytoplasm (H) or with a slight but incomplete posterior bias (I). Scale bar: 10 µm.