Fig. 4. The lng1-1D phenotype is caused by overexpression of LNG1 (At5g15580). (A) Schematic representation of the T-DNA insertion site in the lng1-1D mutant. BAR, Basta resistance (Basta^r). 4x35S denotes four copies of the 35S enhancer. (B) Northern blot analysis of neighboring genes. The bottom panel shows total RNA. (C) LNG1 and its homologs in Arabidopsis and rice. The unrooted phylogenetic tree was generated by a maximum parsimony method using the PHYLIP program. Numbers in the tree indicate bootstrap values. LNG1, At5g15580; LNG2, At3g02170; XP_476397, XP_479072 and AAR01707 include the Oryza sativa. (D-G) Recapitulation analysis with wild type (D), lng1-1D (E), 35S:LNG1 (F) and 35S:LNG2 (G). The plants were photographed 27 days after seeds were sown in the greenhouse.