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Figure 3


Fig. 3. Overexpression of nlk causes the same phenotypes as overexpression of Delta. (A) Morphology of embryos overexpressing nlk or Delta. (a,c,e) Control embryos. (b,d,f,g,h) Embryos injected with nlk mRNA. (i-l) Embryos injected with Delta mRNA. (a,b) Late gastrula, (c,d,i) prism stage, (e-h,j-l) pluteus stage. (vv) Vegetal pole view. (B) Effects of nlk misexpression on the expression of ectodermal, endodermal and mesodermal genes. In situ hybridization was carried out between blastula and gastrula stages. HE (a,g), bhmt (b,h), brachyury (c,i), goosecoid (d,j) and bpnt (e,f,k,l). (b-f) Control embryos. (g-l) Injected embryos.