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Figure 7


Fig. 7. NLK antagonizes the activity of TCF in TOP FLASH assays. (A) Partial sequence alignments between the sea urchin TCF (Pl.TCF) and vertebrate TCF proteins sequences, showing the conservation of the MAPK phosphorylation consensus site. (B) Ectopic expression of NLK downregulates TCF. Embryos were injected at the egg stage and treated with LiCl (30 mmol/l) from the two-cell stage on. Embryos injected with the TOP FLASH construct alone (grey column), plus lithium treatment (dark blue column). Embryos co-injected with the TOP FLASH construct and nlk mRNA (light blue column), plus lithium treatment (green column).