Fig. 10. TrkB expression in OB, and Bdnf action on OBSC-derived neurons. (A-E) Coronal sections from P6 and P21 mice were stained with an antibody to TrkB (A, ML), (B, GL), or double-stained with anti-TrkB and anti-SV2 (C-E), showing that in addition to expression in neuronal cell bodies, TrkB is highly expressed in synaptic terminals at the glomeruli. (F-Q) E13.5-derived OBSC cultures were grown for 15-17 days. Representative fields of TuJ1⁺ neurons (F,G) and synapsin I⁺ neurons (H,I) in control (F,H) and Bdnf-treated (G,I) cultures. Representative fields of GABA⁺/SV2⁺ (J-M) and Th⁺/SV2⁺ neurons (N-Q) in control (J,K,N,O) and Bdnf-treated (L,M,P,Q) cultures. Note that Bdnf-treated neurons show more synapsin I⁺ and SV2⁺ boutons than controls. Similar results were obtained in three experiments. Scale bar in Q: 35 µm for A,B; 25 µm for C-E; 30 µm for F-I; 15 µm for J-Q.