Fig. 2. Gsh2 and Dlx2 expression and interneuron generation in short-term dissociated cultures of E13.5 OB and E13.5 GE. Cell suspensions of E13.5 OB and GE were cultured for 6 days. Representative fields of Gsh2/GAD-positive cells (OB, A-C; GE, G-I) and Dlx2/GAD-positive cells (OB, D-F; GE, J-L) in cultures. Arrows indicate double-labeled cells; arrowheads show GAD⁺ neurons that do not express Gsh2 or Dlx2. (M) Percentages of GAD⁺ neurons over total TuJ1⁺ neurons. (N) Percentages of Gsh2- or Dlx2-expressing GAD⁺ cells relative to total GAD⁺ cells. (O) Percentages of Gsh2- or Dlx2-expressing GAD⁺ cells relative to total Gsh2⁺ or total Dlx2⁺ cells. No (0%) Gsh2⁺ cell coexpressed GAD in OB cultures; Student's t test could thus not be calculated. ^*P<0.05 (OB versus GE). Results are the mean±s.e.m. of data from six cultures in three experiments. Scale bar in L: 25 µm.