Fig. 3. Pax6 expression, BrdU labeling and interneuron generation in the OB. (A-C) E13.5 coronal sections immunostained with a Pax6-specific antibody showed abundant Pax6 expression in the NEZ and in cells of the MZ. (D-F) 52.0±1.1% (n=3) of GAD⁺ cells in culture expressed Pax6 (arrows). Arrowheads show GAD⁺ neurons that do not express Pax6. (G-I) Immunostaining of BrdU-labeled E13.5 mouse sections shows BrdU⁺/GABA⁺ cells (arrows) in the MZ. (J-L) Of cells in culture, 95.4±0.9% (n=3) of GABA⁺ were BrdU⁺ (arrows), indicating that the majority of interneurons are derived from dividing precursors. Similar expression patterns were observed in sections from two to four different mice. Scale bar in C: 60 µm for A; 45 µm for B; 40 µm for C; 35 µm for D-F; 25 µm in J-L; 15 µm in G-I.