Fig. 4. Transplant of GFP-expressing E13.5 OB precursor cells in E13.5 wild-type OB slices. E13.5 OB cell suspensions were transplanted into E13.5 OB slices prepared from wild-type embryos; slices were then cultured for 2 days. (A-C) Representative images of GFP/GABA-positive cells. (D-F) Representative images of GFP/Th-positive cells. (G) Of the total GFP⁺ cells in the slices, 31.7% (n=15) expressed GABA and 12.1% (n=2) expressed Th. Scale bar in C: 25 µm for A-C; 20 µm for D-F.