Fig. 5. Differentiation and maturation of GABAergic, dopaminergic and mitral/tufted neurons in short- and long-term dissociated cultures of E13.5 OB. Cell suspensions were cultured for 6 or 17 days, then single-(A,B) or double-immunostained (C-H). (A,B) Representative images of GABA- and Th-positive cells at 6 days in culture. (C-H) Representative images of MAP2ab/GABA, MAP2ab/Th, and MAP2ab/Tbr1 at 17 days in culture, revealing the morphological maturation of neurons. Images are representative of four to six cultures in three experiments. Scale bar in B: 20 µm for A,B; 30 µm for C-H.