Fig. 7. Differentiation and maturation of E13.5 OB precursor cells expressing GFP in P5-P7 wild-type OB. E13.5 OB cell suspensions were transplanted and mice were analyzed 4 to 10 weeks after surgery. (A) Many granule neurons had a single, large apical dendrite that crossed the ML and extended dendritic branches with spines in the EPL. The granule neurons at the IPL-ML boundary (arrow in A) had two major apical dendrites that also extended branches with spines into the EPL (A,C,D, D inset). Staining with an anti-synaptophysin antibody (E,F) revealed close proximity of dendritic spines to synaptic boutons. (B) As host periglomerular neurons (inset in B shows a periglomerular/glomerular area stained for Th), some transplanted cells oriented their cell bodies horizontally. (G-O) Sections were stained with anti-GAD or anti-GABA antibodies. In some neurons, GAD or GABA and GFP colocalization was best observed in specific areas of the cell body. (P-R) The Th⁺ cell body surrounds the nuclear GFP⁺ signal (arrows). Neuron migration and differentiation was observed in all transplanted mice (n=6). Scale bar in R: 20 µm for A-C; 15 µm for D,G-I; 4 µm for D inset; 10 µm for E,F,J-R.