Fig. 9. Differentiation of OBSC into dopaminergic and mitral/tufted neurons.
(A-D) Representative images of MAP2ab+ and Th+
cells in differentiating E13.5 OBSC cultures. (E) Average percentages
of Th+ neurons relative to total MAP2ab+ neurons.
Results are the mean±s.e.m. of data from four cultures in two
experiments. Scale bar: in D, 40 µm. (F) Average percentages of
Tbr1+ vs total MAP2ab+ neurons. Bdnf-treated cultures
had 26% more Tbr1-labeled cells than controls (not statistically significant).
Results are the mean±s.e.m. of data from four to six cultures in three
experiments. (G-J) Representative images of MAP2ab+ and
Tbr1+ cells. Arrows show Tbr1+ nuclei in the neurons.
Scale bar in J: 50 µm.