Fig. 3. Activation and repression by DL variants visualized by double labeling. (A-D) TWI protein expression was monitored by antibody staining (red, left), while zen mRNA expression was monitored by in situ hybridization (blue, right). Sagittal, confocal sections of the embryos are oriented with anterior on the left and the dorsal side at the top. Maternal genotypes are described in the Materials and methods.