Fig. 1. Developmental delay of M(3)67C/+ discs. (A) Duration of the larval period in hours in wild-type (blue line), esg-Gal4; M(3)67C/+ (broken black line), en-Gal4; M(3)67C/+ (broken green line) and ci-Gal4; M(3)67C/+ (broken red line) flies. (B,B') Second instar wing disc from a esg-Gal4; M(3)67C Ubi-GFP FRT2A/+ larvae. The disc in B is triple labelled for GFP (green), wg (red) and PH3 (blue). All cells contain GFP expression, those in mitosis are scattered and wg expression is restricted to the region around the DV border. (C) esg-M⁺ disc. All the cells have lost the GFP marker (the green label corresponds to adepithelial cells which are of mesodermal origin that do not express esg). wg expression is more advanced than in the disc in B.