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Figure 3


Fig. 3. Analysis of differentiation markers in K5Cre Jag1flox/flox, K14CreER Jag1flox/flox and K14NICD{Delta}OPER skin. (A-O) Back skin of Jag1flox/flox (A,H,I,L,M), K5Cre Jag1flox/flox (B,D,J,K,N,O) and 4OHT-treated K14NICD{Delta}OPER (C,E,F,G) mice stained with antibodies to K10 (A-D), CDP (E,H-K), hair keratins (AE13) (F) or trichohyalin (G,L-O). (H,J,L,N) Sections DAPI counterstained (blue). (P-W) Whole mounts of Jag1flox/flox (P-S), K5Cre Jag1flox/flox (T-V) and K14CreER Jag1flox/flox (W) tail epidermis immunolabelled for K14 (P,T,S,W) and CDP (Q,U) or stained with Nile Red (green) and DAPI (blue) (R,V). Dashed lines outline the hair follicles and sebaceous glands. Scale bars: 50 µm in D; 100 µm in A-C,E-W. A, anagen follicles; T, telogen follicles.