Fig. 3. Analysis of differentiation markers in K5Cre Jag1flox/flox,
K14CreER Jag1flox/flox and K14NICD
OPER skin.
(A-O) Back skin of Jag1flox/flox (A,H,I,L,M), K5Cre
Jag1flox/flox (B,D,J,K,N,O) and 4OHT-treated
K14NICD
OPER (C,E,F,G) mice stained with antibodies to K10
(A-D), CDP (E,H-K), hair keratins (AE13) (F) or trichohyalin (G,L-O).
(H,J,L,N) Sections DAPI counterstained (blue). (P-W) Whole mounts of
Jag1flox/flox (P-S), K5Cre Jag1flox/flox (T-V) and
K14CreER Jag1flox/flox (W) tail epidermis immunolabelled for K14
(P,T,S,W) and CDP (Q,U) or stained with Nile Red (green) and DAPI (blue)
(R,V). Dashed lines outline the hair follicles and sebaceous glands. Scale
bars: 50 µm in D; 100 µm in A-C,E-W. A, anagen follicles; T, telogen
follicles.