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Figure 3


Fig. 3. Lef1 is not required for molecular markers of hypothalamus identity or AP patterning. White circles outline posterior hypothalamus. (A,C,E) Uninjected embryos. (B,D,F) Embryos injected with 2 ng of lef1 MO. (A,B) nk2.1a expression in the entire hypothalamus is unaffected in lef1 morphants at 30 hpf. (C,D) rx3 is still expressed in the anterior hypothalamus in lef1 morphants at 30 hpf. (E,F) emx2 is still expressed in the posterior hypothalamus of lef1 morphants at 30 hpf.