Fig. 4. Lef1 is required for proneural and neuronal gene expression in the posterior hypothalamus. White circles outline posterior hypothalamus in whole-mount views, and black ovals outline posterior hypothalamus in cross-sections. Lines in whole-mount images show plane of corresponding cross-sections. (A-D) Expression of sox3 is absent in the posterior hypothalamus of lef1 morphants at 24 hpf. (E-H) Expression of zash1a is absent in the posterior hypothalamus of lef1 morphants at 28 hpf. (I-P) Expression of dlx2 and isl1 are absent in the posterior hypothalamus of lef1 morphants at 30 hpf. (Q-T) Expression of ngn1 and olig2, which are expressed in the posterior tuberculum, is unaffected in the posterior tuberculum of lef1 morphants.