Fig. 2. Expression of Cv2 analyzed with nlacZ-knocked-in mice. (A,B) At E10.5, lacZ staining was observed in the dorsal midline of the neural tube (arrowhead), migrating neural crest (nc), head mesenchyme, trigeminal ganglion (t), otic vesicle (o), para-aortic region and mesonephros (mn). (B) Transverse section at position shown in A. (C,D) E11.5 embryo. Strong staining appeared in the presumptive vertebral body (arrow) and arch regions (arrowhead). Black arrowhead indicates the dorsal midline of the neural tube. (E-G) At E14.5, the staining was observed in the vertebral body (vb), vertebral arch (va), dorsal root ganglion (drg), part of the neural tube, lung (l), heart (h) and ribs (r). (E) Sagittal section of the trunk region, showing that intervertebral discs (ivd) are lacZ negative. (G) Higher magnification of the neural tube and vertebra. (H-K) Developing kidneys. The condensed nephrogenic mesenchymes were stained with X-gal at E14.5 (H). At P0 (I-K), the staining was observed in the condensed nephrogenic mesenchymes (arrowheads) surrounding the distal tip of the collecting duct, and in the comma-shaped bodies (cb).