Fig. 6. Kidney defects in the Cv2 mutant. (A-D) External appearances (A,B) and longitudinal sections (C,D; along the longest axis) of the kidneys at P0. Scale bars: 500 µm. Reduced kidney size was observed in the Cv2^-/- mutant (B,D). (E,F) HE staining showed no obvious morphological defects of the renal corpuscle in the Cv2^-/- mutant kidney at P0 (F). (G,H) The kidney was smaller in the Cv2^-/- mutant (H) at E14.5. (I,J) Whole-mount in situ hybridization of Pax2 expression in the control (I) and mutant (J) kidneys. The Pax2-positive condensed mesenchymes were formed in the mutant, but the number was reduced (K; ^*P<0.05, t-test). (L,M) WT1-positive condensed mesenchymes and glomerular podocytes (green) and E-cadherin-positive collecting ducts (red) are shown in whole-mount immunostaining. The general architecture of the kidney was largely unaffected in the Cv2^-/- mutant (M).