Fig. 8. S697A mutation does not impair neurite outgrowth and proliferation/survival of cultured ENCCs. (A) Neurite outgrowth of cultured ENCCs with GDNF stimulation. ENCCs were dissociated from E11.5 midgut and cultured for 3 days in the absence or presence of GDNF. Neurites were visualized by immunostaining with anti-PGP9.5 antibody. (B) Quantitative analysis of cultured ENCCs. After 3 days of culture in the absence or presence of GDNF, anti-PGP9.5-positive ENCCs were counted. The mean was calculated from ten views of each of three cultures.