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Figure 10


Fig. 10. Hyperdorsalization results from radialized vegetal microtubule array. (A) Embryos treated with 20 mmol/l BDM for 20 minutes during the first cell cycle displayed a range of conjoined-twin and radialized hyperdorsal morphologies. (B) A normal vegetal-cortical microtubule array, seen from the side in a whole-mount embryo immunostained for {alpha}ß-tubulin via confocal microscopy. The specimen is oriented with the animal pole up, sperm entry point to the right, and thus prospective dorsal to the left. (C) A vegetal-cortical microtubule array in a BDM-treated sibling displaying the extreme hyperdorsal phenotypes seen in A. The specimen orientation is the same as that for B.