Fig. 2. max-2 is a single gene that includes the two predicted ORFs F18A11.4 and Y38F1A.10. (A) A diagram of a genomic region on the distal right arm of chromosome II (LGII) that contains the max-2 gene. Cosmid and YAC coverage in this region are diagrammed with black bars. The boxed region of the schematic is enlarged and shows the region that contains the two predicted ORFs F18A11.4 and Y38F1A.10 (boxed region is not to scale). (B) A diagram of the max-2 genomic organization (upper) and the minigene construct (lower) used for the rescue experiments in (C). The boxed area indicates the region deleted in the max-2(nv162) allele, and the asterisk indicates the site of the point mutation identified in the max-2(cy2) allele. (C) max-2 mutants are rescued by injecting the rescue construct depicted in B. The graph shows the percentage of the DD and VD motor commissures that fail to reach the dorsal cord (DC). Two independent transgenic lines expressing the minigene construct znex130-131[Pmax-2::max-2(cDNA)] are shown here; non-transgenic siblings that lack the transgene are also shown for znex131. (D) max-2(cy2) and max-2(nv162) show similar severity in the defects of dorsal guidance of commissural axons. Injecting double-stranded RNA (dsRNA) of the max-2 gene causes less severe axon guidance defects that are otherwise similar to the max-2 mutants. The numbers (n) of animals used for each experiment are shown; bars represent the standard error (C,D).