Fig. 3. Genetic and physical interactions between Smg and the CCR4-NOT complex of deadenylation. (A) Genetic interaction between smg and twin. Females of the indicated genotypes were crossed with wild-type males, and hatched and unhatched embryos were scored. (B) PAT assays of nos and control sop mRNAs showing a wild-type pattern of nos mRNA poly(A) tails in embryos from smg¹/+ or twin⁸¹¹⁵/+ females and a stabilization of nos mRNA with longer poly(A) tails in embryos from smg¹/+ twin⁸¹¹⁵/+ double heterozygous females; poly(A) tails were still longer in embryos from double homozygous smg¹ twin¹²²⁰⁹ mutant females (right panel). Females were crossed with identical males. (C) Co-immunoprecipitation of CAF1 protein with Smg in 0-3 hour embryo extracts. Proteins were immunoprecipitated with anti-Smg (Smg IP) or rabbit serum (mock IP) either in the presence or the absence of RNase A. Bound proteins were detected by western blots with anti-Smg or anti-CAF1. Extract before IP was also loaded.