Fig. 5. Characterization of embryonic phenotypes caused by overexpression of osk with UASp-osk-K10. (A,B) Cuticle of embryos from UASp-osk-K10/+; nos-Gal4/+ females were prepared and classified from their defects (n=155). Examples of the different phenotypes are shown in B. Two different examples of strong head defects (25%) are shown: head replaced by a hole (middle top panel), no head structures (middle bottom panel). The phenotype of mirror posterior duplication (51%) is also shown (bottom panel). (C) Immunostaining of embryos from wild-type or UASp-osk-K10/+; nos-Gal4/+ females with anti-Nos antibody, showing that Nos protein accumulates in the whole embryo when osk is overexpressed ubiquitously. Anterior is oriented toward the left.