Fig. 7. Presence of Smg and of the CCR4-NOT complex in discrete cytoplasmic foci related to P bodies. Immunostaining of wild-type syncytial blastoderm embryos (90-110 minute development) with anti-CAF1, anti-CCR4, anti-Pacman or anti-Dcp1, costained with anti-Smg and DAPI (not shown). Pacman and Smg are exclusively cytoplasmic, whereas CAF1, CCR4 and Dcp1 are present in low amounts in nuclei, in addition to their cytoplasmic distribution. All proteins show a diffuse cytoplasmic distribution and accumulation in cytoplasmic foci variable in size. Arrows indicate medium size foci where either CAF1, CCR4 or Pacman colocalize with Smg. Arrowheads indicate large size Smg foci that do not contain CAF1, CCR4 or Pacman, but do contain Dcp1.