Fig. 7. Markers of cardiomyocyte differentiation are down regulated in Hand1-overexpressing embryos. RNA in situ hybridisation analysis of Anf and Wnt11 in control embryos (A,C,E,G,I) and moderate phenotype Hand1-overexpressing embryos (B,D,F,H,J) at E9.5. Sagittal sections of the control (C,I) and Hand1-overexpressing hearts (D,J). Boundary of Anf expression in proximal outflow tract of overexpressing embryos is depicted by a broken red line in F. Wnt11 in distal outflow tract (black and white arrowheads) is downregulated in overexpressing embryos (H,J) compared with control embryos (G,I). at, atrium; co, control; do, distal outflow tract; lv, left ventricle; oe, overexpression; oft, outflow tract; po, proximal outflow tract. Scale bar: 100 µm in A.