Fig. 8. Primary heart field markers are downregulated in Hand1-overexpressing embryos. RNA in situ hybridisation analysis of Nkx2.5 and Gata4 in control embryos (A,C,E,G,I,K,M,O) and moderate phenotype Hand1-overexpressing embryos (B,D,F,H,J,L,N,P) at E9.5. (I-P) Sagittal sections of the control (I,K,M,O) and Hand1-overexpressing (J,L,N,P) hearts. (Q) Quantitative real-time PCR analysis on RNA extracted from control or severe phenotype overexpression embryos for an array of cardiac markers. Means and standard deviation bars with 95% confidence limits are shown. ^*P<0.05, ^**P<0.01. at, atrium; co, control; lv, left ventricle; oe, overexpression; oft, outflow tract; rv, right ventricle. Scale bar: 100 µm in A.