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Figure 9


Fig. 9. Over-proliferation of cardiac precursors in the distal OFT of Hand1-overexpressing embryos. Sagittal (A-D) and frontal (E-H) sections through the distal OFT region of control or Hand1-overexpressing embryos at E9.5. Immunofluorescence with antibody specific to phospho-histone H3 ({alpha}-PH3) to mark proliferating cells (A,C,E,G). (B,D,F,H) Bis-benzamide counterstaining (DNA) of nuclei. White arrowheads in C and G indicate areas of proliferating cells. co, control; oe, over-expression.