Fig. 3. let-7 mir-84 adults express gfp reporters associated with molting. (A-D) Animals were cultivated at 25°C following release from starvation at the early L1 stage. (A) Prevalence of fluorescence from mlt-10p::gfp-pest, as detected with a Zeiss SV-6 microscope. Each sample contained 52 or more animals. Data were collected in three experiments to cover all time points. Error bars indicate the standard deviation from the mean in cases where two populations of animals were observed at the same time point. (B) Fluorescence from mlt-10p::gfp-pest in adults cultivated for 56 hours. (C) Western blot showing GFP in protein extracts from late L4-stage or gravid adults cultivated, respectively, for 41 or 56 hours. Each strain carried the mgIs49[mlt-10p::gfp-pest] transgene. Levels of ß-tubulin provide a loading control. (D) Fluorescence from nas-37p::gfp-pest in adults.