Fig. 4. Inactivation of precocious heterochronic genes prevents the supernumerary molt of let-7 mir-84 mutants. Synchronized populations of L1 larvae were fed bacteria expressing dsRNA corresponding to the indicated genes and cultivated at 25°C. Animals in the P₀ generation were scored for fluorescence from mlt-10p::gfp-pest 56 hours later and then for viability the next day. To score F₁ animals, progeny were collected, synchronized as L1 larvae, and then fed the appropriate bacterial clone for an additional 55 hours.