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Figure 5


Fig. 5. bchs mutations suppress bristle defects of rab11 mutants. (A) Portions of posterior abdomens from indicated genotypes. In control, each socket contains a bristle (microchaete), but in rab11ex1/rab1193Bi many sockets are empty. Mutation of one copy of bchs (bchs17/+;rab11ex1/rab1193Bi) restores many bristles, while mutation of both copies of bchs (bchs17/bchs12;rab11ex1/rab1193Bi) restores all bristles. Arrowheads indicate bristle-filled or empty sockets in the last row of the third abdominal tergite (segment). (B) Portions of posterior scutellum from indicated genotypes. Posterior scutellar bristles are shortened in rab11ex1/rab1193Bi. Bristle length is restored partially by one mutant copy of bchs (bchs17/+;rab11ex1/rab1193Bi) and more fully by homozygosity for bchs (bchs17/bchs12;rab11ex1/rab1193Bi). (C) Quantitation of abdominal bristle loss. The fraction of sockets containing bristles was calculated for the last row of abdominal tergites 2, 3 and 4. Error bars are s.e.m. Df is Df(2L)cl7, a chromosome deleted for bchs. Top panel: reduction of bchs gene dosage suppresses bristle loss of rab11ex1/rab1193Bi. n≥10 for each genotype, P<0.0001 for all genotypes compared to rab11ex1/rab1193Bi. Bottom panel: reduction of bchs gene dosage suppresses bristle loss of rab1193Bi homozygotes. n≥10 for each genotype, P<0.0001 for all genotypes compared to rab1193Bi/rab1193Bi. Scale bars: 30 µm in A; 50 µm in B.