Fig. 5. bchs mutations suppress bristle defects of rab11
mutants. (A) Portions of posterior abdomens from indicated
genotypes. In control, each socket contains a bristle (microchaete), but in
rab11ex1/rab1193Bi many sockets are empty.
Mutation of one copy of bchs
(bchs17/+;rab11ex1/rab1193Bi)
restores many bristles, while mutation of both copies of bchs
(bchs17/bchs12;rab11ex1/rab1193Bi)
restores all bristles. Arrowheads indicate bristle-filled or empty sockets in
the last row of the third abdominal tergite (segment). (B) Portions of
posterior scutellum from indicated genotypes. Posterior scutellar bristles are
shortened in rab11ex1/rab1193Bi. Bristle length
is restored partially by one mutant copy of bchs
(bchs17/+;rab11ex1/rab1193Bi) and
more fully by homozygosity for bchs
(bchs17/bchs12;rab11ex1/rab1193Bi).
(C) Quantitation of abdominal bristle loss. The fraction of sockets
containing bristles was calculated for the last row of abdominal tergites 2, 3
and 4. Error bars are s.e.m. Df is Df(2L)cl7, a chromosome
deleted for bchs. Top panel: reduction of bchs gene dosage
suppresses bristle loss of rab11ex1/rab1193Bi.
n
10 for each genotype, P<0.0001 for all genotypes
compared to rab11ex1/rab1193Bi. Bottom panel:
reduction of bchs gene dosage suppresses bristle loss of
rab1193Bi homozygotes. n
10 for each genotype,
P<0.0001 for all genotypes compared to
rab1193Bi/rab1193Bi. Scale bars: 30
µm in A; 50 µm in B.