Fig. 8. Bchs and Rab11 partially overlap within the NMJ. (A) Rab11 positive puncta are present in the motor axon (arrowhead), synaptic boutons (arrow) and muscle (star). The number and brightness of Rab11 puncta are reduced in rab11^ex1/rab11^93Bi hypomorph (right panels) compared with control (left panels). Confocal stacks through NMJs at muscle 4 are shown. (B) rab11^ex1/rab11^93Bi mutants have reduced levels of Rab11 protein compared with controls. Anti-Elav serves as control for protein loading. (C) Single confocal slice through muscle 4 NMJ of third instar larva, double labeled for Rab11 (green) and Bchs (red), reveals significant overlap. Anti-HRP (blue) outlines neuronal membrane. Scale bars: 5 µm.