Fig. 9. Bchs and Rab11 partially co-fractionate in membrane preparations. Post-nuclear fractions of Canton S head extracts were fractionated on a 10-30% sucrose gradient and characterized in Adolfsen et al. (Adolfsen et al., 2004). (A) Synaptic vesicle fractions were detected with anti-Synaptotagmin1 (Syt1) antibody. Bchs and Rab11 localize to partially overlapping fractions (15-18). Unlike Rab11, Bchs was not present in the plasma membrane (fraction 1; containing ROP and syntaxin) or Syt1-positive synaptic vesicle fractions. Fractions positive for the plasma membrane marker Syx1A and synaptic vesicle marker n-Syb, as published in Adolfsen et al. (Adolfsen et al., 2004), are marked.