Fig. 10. Analysis of the elc kis-T1 double mutant. (A-C) Comparison of kis-T1 (A), elc (B) and kis-T1 elc (C) mutant rosette leaves. (D-F) Comparison of kis-T1 (D), elc (E) and kis-T1 elc (F) mutant trichomes. Note that the cluster frequency is strongly enhanced in the double mutant. (G-I) Comparison of kis-T1 (G), elc (H) and kis-T1 elc (I) mutant epidermal pavement cells. Cell size is reduced in the double mutant. (J-L) Comparison of kis-T1 (J), elc (K) and kis-T1 elc (L) sections of rosette leaves. The integrity of the leaf is strongly disturbed in the double mutant. (M-O) Higher magnification of propidium iodide-stained leaf sections from kis-T1 elc double mutants. Multinucleated cells are frequently observed in the kis-T1 elc double mutants. Nuclear anomalies like nuclear bridges (arrowhead, M) and high numbers of nuclei per cell (N) were found frequently. In very rare cases, multinucleated stomata with incomplete cell walls were observed (arrowhead, O).