Fig. 4. Molecular analysis of the ELC gene. (A) Schematic of the chromosomal region of the ELC gene. The fragment used for the rescue experiments is indicated. (B) The chromosomal region of the ELC gene shows two insertions in the mutant. (C) The insertion in the ELC gene results in a truncated protein. A sequence alignment of the wild-type and mutant proteins and a schematic highlighting their domain structure are shown. (D) Sequence alignment of ELC (At3g12400) and ELC-like (At5g13860) from Arabidopsis thaliana (At), ELC from Oriza sativum (Os) (BAD28453), yeast Vps23 (Sc) (Af004731), human TSG101 (Hs) (U82130) and Drosophila TSG101 (Dm) (NM_079396). Letters in a black background indicate identity, dark gray backgrounds indicate strong similarity and a light gray background indicates weak similarity. (E) Schematic of the protein domain arrangement of ELC, TSG101 and Vps23. The characteristic absence of a cysteine conserved in UBC domains (star) is depicted below.