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Figure 5


Fig. 5. PLL1 overexpression in clv mutants blocks differentiation. (A-P) clv2-1 (A,B,E,F,I,J) and clv3-2 (C,D,G,H,K-P) plants carrying P35S:PLL1 (A-D,N-P), PER:PLL1 (E-H) or no transgene (I-M) revealed massive accumulation of stem cells and a block of differentiation resulting from PLL1 overexpression. (A,C,E,G,I,K,M-O) At 10 days after germination; (B,D,F,H,J,L) 15 days after germination; (P) 6 days after germination. PCLV3:GUS (O) and PWUS:GUS (P) were expressed throughout the apices of clv3-2 P35S:PLL1 plants, indicating stem-cell character. Scale bars: 250 µm.