Fig. 4. SUF4 expression, and its effect on flowering time and gene expression in FRI and AP-mutant backgrounds. (A) RT-PCR analysis of SUF4 in the early stages of development and in various tissues. Expression of SUF4 and FLC at 2-12 days after germination (DAG), and in shoots (S), roots (R), leaves (L) and flowers (F). (B) Effect of genotype and vernalization on SUF4 and FLC expression, as determined by RT-PCR. (C) RT-PCR analysis of FRI expression in wild-type (+) and suf4-mutant (-) backgrounds. (D) RT-PCR analysis of FLC expression in the indicated backgrounds with wild-type SUF4 (+) or mutant suf4 (-). (E) Effect of suf4 mutations on flowering time. Bars represent the total number of leaves (rosette and cauline) formed by the primary shoot apical meristem. Black bars represent the indicated genotypes with wild-type SUF4; white bars represent the indicated genotypes with the suf4 mutation. Error bars represent the s.d.