Fig. 1. Increased IIS produces lipid-droplet accumulation defects in nurse cells. (A,B) Nile Red-positive small neutral lipid droplets (red) accumulate in wild-type nurse cells, particularly in a perinuclear region (arrowheads in B), which appears to co-localize with the endoplasmic reticulum (Teixeira et al., 2003). By contrast, non-GFP-labelled (not green) Pten mutant cells (D-I) contain large aggregated lipid droplets of up to 15 µm diameter (arrowheads in E,H; shown at high magnification in F,I) and contain far fewer small droplets. (C) This phenotype is completely suppressed in Pten mutant animals rescued by a specific combination of Akt alleles (Stocker et al., 2002). (J-O) In some egg chambers, overexpression of Dp110 in nurse cells induces sporadic formation of superficial, elongated lipid-containing structures close to the plasma membrane (arrowheads in K,N). The profile of these cells is outlined by intense GFP expression in overlying somatic stretched follicular cells (green in L,O; see also deeper section in Fig. 3C). Scale bars: 40 µm in A-C,F,I; 40 µm in E for D,E; 40 µm in G for G,H; 40 µm in J for J,K,L; and 40 µm in M for M,N.O.