Fig. 2. Cytoplasmic activated Akt is greatly increased in Pten-mutant nurse cells. (A,B) Nurse cells from wild-type egg chambers show a modest accumulation of activated P-Akt (blue) in the cytoplasm. The actin cytoskeleton of these cells is labelled with TRITC-phalloidin (red). (E-L) Non-GFP-labelled (not green in H,L) Pten mutant cells have no obvious cortical cytoskeletal abnormalities (G,K), but contain much higher levels of cytoplasmic P-Akt (F,J). (C,D) Elevated levels of P-Akt are observed in the cytoplasm of mutant cells but not in nuclei, which are stained with propidium iodide (red) in this egg chamber. The positions of Pten mutant nuclei are marked in red in D. (M-P) Nurse cells overexpressing Dp110 produce increased cell-surface P-Akt (N), co-localizing with cortical actin (O). Intensely GFP-positive nuclei of peripheral Dp110-overexpressing stretched follicle cells overlying the nurse cells are also observed in this image (P, arrowheads). Scale bar: 40 µm in A for A,B; 40 µm in D for C,D; 40 µm in E for E-H; 40 µm in I for I-L; and 40 µm in M for M-P.