Fig. 1. CP2L1 expression in ducts of exocrine glands and kidney. (A,B,D-K) LacZ expression from the Cp2l1 trap locus in the duct of submandibular and sublingual glands (A), isolated SMG (B), parotid and lachrymal glands (D) and nasal gland (E) at E16; in the glandular ducts in olfactory epithelium (F) and ducts of the mammary gland (G), male reproductive system (I), endolymphatic sac (J) and lung (K; right, Cp2l1^+/tra; left, wild-type control) at birth; and in eccrine glands in the palm of the adult (H; right, Cp2l1^+/tra; left, wild-type control). (C) Co-detection of endogenous CP2L1 protein (magenta) by an antiserum to CP2L1 (As2375) and keratin 7 (green) in the duct of the SMG. (L) Genotyping of Cp2l1 trap locus mice. The gene-trap vector pLSAßgeo was inserted into the second intron of the Cp2l1 locus. Amplification primers were designed as shown in the lower portion of L. This set of primers could distinguish between the wild-type allele (+) and the Cp2l1 trap allele (tra). (M) Immunohistochemical detection of CP2L1 protein in a kidney section using As2375. Although this serum reacted with unidentified proteins in the cytoplasm of glomerulus cells, CP2L1 protein in the nucleus was not detected in Cp2l1^tra/tra mice. (N) Survival rate of Cp2l1^+/tra (n=56) and Cp2l1^tra/tra mice (n=48). (O) Growth curve of each genotype. P0-P21, postnatal day 0-21. Scale bar: 25 µm in C,M; 100 µm in J. LG, lachrymal glands; PG, parotid glands.