Fig. 3. Abnormalities in gene expression in the SMG of Cp2l1^tra/tra mice. (A-D,G) In situ hybridization analysis of the SMG at birth. Expression of the duct-specific genes keratin 7 (A), Gk-6 (B), Fxyd2b (C), Scnn1b (D) and keratin 19 (G) was abnormal in Cp2l1^tra/tra mice compared with Cp2l1^+/tra mice. (E) Expression of CP2L1 and keratin 7 examined in adjacent sections of the nasal gland. In the duct of the nasal gland, Cp2l1 (lacZ expression; left) was coexpressed with keratin 7 (right) in control Cp2l1^+/tra mice (upper panels). In Cp2l1^tra/tra mice (lower panels), based on the morphology and the expression of Cp2l1 (left), the duct was formed, although keratin 7 (right) was not expressed. (F) Comparison of mRNA expression levels in the newborn SMG of Cp2l1^+/tra and Cp2l1^tra/tra mice by quantitative RT-PCR. ^*P<0.005. Scale bars: 100 µm.